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Sterile bio line 1: bio chaff → bacteria → bacterial sludge → enriched sludge → mutagen (every step inside a Sterile Cleanroom)

Chain id: uv_bio1 · Tags: Infinite source, Unverified

Era setup: UV · Abydos

Plan modules: Sterile bio lines

Tips: 5, 1 unverified

Machines

HV Brewery · 15 s · 480 EU/t · Sterile CleanroomHV
Oak 14 bio chaff · 1 B distilled waterBio chaffDistilled water1
up to 512 EU/t15 s
BacteriaBacteria 1 B1 B
gtceu:hv_brewery
EV Chemical Reactor · 30 s · 1920 EU/t · Sterile CleanroomEV
BacteriaBacteria 1 B1 BOak 11 B biomassBiomass1 B
up to 2 048 EU/t30 s
Bacterial SludgeBacterial sludge 1 B1 B
gtceu:ev_chemical_reactor
Brewery (any tier) · 6.4 s · 4 EU/t · Sterile CleanroomHV
Bacterial SludgeBacterial sludge 1 B1 BOak 11 uranium dust (238) OR 1 tiny naquadria dustUranium 238 dustTiny naquadria dust1
up to 512 EU/t6.4 s
Enriched Bacterial SludgeEnriched bacterial sludge 1 B (2 B with naquadria)1 B
gtceu:hv_brewery
IV Distillery · 5 s · 7680 EU/t · circuit 1IV
Enriched Bacterial SludgeEnriched bacterial sludge 1 B (2 B with naquadria)1 B
up to 8 192 EU/t5 s
Mutagen BucketMutagen 100 mB per B of enriched sludge100 mB
gtceu:iv_distillery

Flow

How to build

  1. Drawers first, one per solid input: bio chaff (Keep Exact 64), uranium dust (238) or tiny naquadria dust. Drums first for fluids: distilled water, biomass, bacteria, sludge, enriched sludge, mutagen. Everything in this chain sits INSIDE the Sterile Cleanroom: use the cleanroom passthrough hatches or put the machines in the room and run Pipez fluid pipes through the wall. — functionalstorage:oak_1, gtceu:bio_chaff, gtceu:uranium_dust
  2. Bacteria brewery: a router from the chaff drawer (Item Filter, Robot Arm cover Keep Exact 4) and a Pipez fluid pipe from the distilled water drum. 4 chaff + 1 B water → 1 B bacteria every 15 s at 480 EU/t. Output drum for the bacteria. — gtceu:hv_brewery, gtceu:bacteria_bucket
  3. Sludge reactor: bacteria drum and biomass drum each by their own Pipez fluid pipe, 1 B + 1 B → 1 B bacterial sludge every 30 s at 1920 EU/t. Keep the biomass drum filled before you open the bacteria valve. — gtceu:ev_chemical_reactor, gtceu:bacterial_sludge_bucket
  4. Enrichment brewery (second brewery, its own drawer of uranium dust (238)): 1 dust + 1 B sludge → 1 B enriched sludge in 6.4 s at 4 EU/t. With tiny naquadria dust the output doubles to 2 B. — gtceu:hv_brewery, gtceu:uranium_dust, gtceu:enriched_bacterial_sludge_bucket
  5. Distillery: circuit 1 selects the mutagen output, 1 B enriched sludge → 100 mB mutagen in 5 s at 7680 EU/t. Put the mutagen in its own drum with a fluid pipe to the mixer of bio line 2. — gtceu:iv_distillery, gtceu:mutagen_bucket
  6. Easy to miss: the drums need to hold 40 B of enriched sludge buffer for one batch of medium. A steel drum holds 32 B, so use two drums per fluid for this buffer, and never merge sludge and enriched sludge in one drum. — gtceu:mutagen_bucket, functionalstorage:oak_1

Tips

  • Why this chain: wetware circuits (the UV circuit and the UHV circuit) need stem cells and sterilized growth medium, and both come from this line. Verified in gtsrc GrowthMediumRecipes: bacteria = brewery, 4 bio chaff + 1000 mB distilled water, 300 ticks at VA[HV]; bacterial sludge = chemical reactor, 1 B biomass + 1 B bacteria, 600 ticks at VA[EV]; enriched sludge = brewery, 1 uranium dust (238) (or 1 tiny uranium 235 dust, or 1 tiny naquadria dust for 2 B) + 1 B sludge, 128 ticks at 4 EU/t; mutagen = distillery, 1 B enriched sludge → 100 mB, circuit 1, 100 ticks at VA[IV]. All of them carry the STERILE cleanroom tag.
  • Sterile Cleanroom (quest text, kjs lang): the Sterilizing Filter Casing replaces the normal filter casings and turns the cleanroom into a Sterile Cleanroom; normal cleanroom recipes do NOT run in a sterile one, so keep a normal cleanroom for the circuit assemblers and a second sterile one for this line. Sterilizing Filter Casing (gtsrc CraftingRecipeLoader, crafting table, rows large PBI fluid pipe + ZPM emitter + large PBI fluid pipe / item filter + blacklight + item filter / ZPM motor + tritanium frame + naquadah alloy rotor): the cost is ZPM parts per casing, and the smallest cleanroom pattern has 8 filter spots in its ceiling (Unverified for larger rooms). The Sterile Cleaning Maintenance Hatch that would run sterile recipes outside a cleanroom is a UIV Assembly Line item (needs a UEV hull), so it is not an option in this era.
  • Yield warning: the distillery gives only 100 mB of mutagen per 1 B of enriched sludge. One batch of the raw growth medium (4 B) needs 4 B of mutagen = 40 B of enriched sludge = 40 B of sludge, i.e. 40 B of biomass and 40 B of bacteria (160 bio chaff) plus 20 tiny naquadria dust (naquadria route, 2 B per dust) or 40 uranium dust (238). The mutagen is by far the slow part of the wetware line; scale the brewery and the reactor in parallel (Large Chemical Reactor or the Chemical Plant are the multiblock options). A pack Chemical Plant recipe makes mutagen directly (mutagen_skip: 1 naquadria dust + 9 B biomass + 9 B bacteria → 1.8 B mutagen, 12 ticks) but at UEV power, so it is a later upgrade.
  • Sub-steps people skip: (1) distilled water and bio chaff come before everything: biomass and bio chaff sources are in the HV/EV chemistry chains, check that your biomass line exists; (2) the sludge reactor returns nothing but the sludge: keep Keep Exact 1000 mB on the biomass and bacteria drums with Pipez fluid pipes; (3) uranium dust (238) is consumed, 1 per B of sludge, so find a uranium source early (Unverified which pack route); (4) one dedicated brewery for the bacteria and a second for the enriched sludge, never share a machine between two recipes.
  • Unverified the bio chaff and biomass sources (bio chaff from the Large Farm / composter chain is the GT default), the sterile cleanroom footprint in this pack, and the exact count of filter casings the sterile cleanroom needs.

Bill of materials

Item Qty
gtceu:hv_brewery 2
gtceu:ev_chemical_reactor 2
gtceu:iv_distillery 1
gtceu:cleanroom 1
gtceu:sterilizing_filter_casing 8
functionalstorage:oak_1 10
modularrouters:modular_router 6
gtceu:lv_robot_arm 6
gtceu:item_filter 6
pipez:item_pipe 16
pipez:fluid_pipe 6
gtceu:steel_drum 5

Needs / Feeds

  • Main source for: gtceu:mutagen_bucket, gtceu:bacteria_bucket, gtceu:bacterial_sludge_bucket, gtceu:enriched_bacterial_sludge_bucket, gtceu:sterilizing_filter_casing